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In animal smoke exposure models, NLRP3 or GSDMD gene knockout mice exhibit reduced pulmonary inflammatory infiltration, decreased airway remodeling, and structural damage, further supporting the pathogenic role of pyroptosis in COPD progression at the in vivo level (96, 97)

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The endogenous antioxidant enzymatic system has evolved to control the detrimental effects of free radicals in the ear, including increased levels of antioxidant enzymes, such as copper/zinc and manganese superoxide dismutases (SOD1 and SOD2, respectively), catalase, GSH-related enzymes (glutathione peroxidases (GPxs), glutathione transferases (GSTs), and glutathione reductase), and peroxiredoxins (PRDXs)